TRAX Data Quality Report
Date: Tuesday January 18, 2022
Run mode: tRNA fragments
Summary
Passed Sequencing read merging rate > 60.0% (adapt1_sp.pdf)
Passed Mappable reads > 200000 (TestTrnas-mapinfo.pdf)
Warning Mapping rate > 65.0% (TestTrnas-mapinfo.pdf)
Warning tRNA read share > 5.0% (TestTrnas-typecounts.pdf)
Passed rRNA read share < 35.0% (TestTrnas-typecounts.pdf)
Passed Reads mapping to unannotated regions < 35.0% (TestTrnas-typecounts.pdf)
Passed Read length average < 40 bases (TestTrnas-readlengths.pdf)
Passed >= 70.0% of reads between 15 and 50 bases (TestTrnas-readlengths.pdf)
Passed >= 50.0% of tRNAs with more than 20 reads (TestTrnas-tRNAcounts.txt)
Passed DESeq2 size factor differences < 3.0x (TestTrnas-SizeFactors.txt)
Sequencing read merging rate > 60.0%
| Status | Sample | Merging Rate |
|---|
| Passed | SRR029131 | 95.36% |
| Passed | SRR029124 | 99.61% |
| Passed | SRR207111 | 97.74% |
| Passed | SRR207116 | 98.72% |
back to Summary
Mappable reads > 200000
| Status | Sample | Read Count |
|---|
| Passed | SRR029131 | 3547303.00 |
| Passed | SRR029124 | 4074720.00 |
| Passed | SRR207111 | 6061229.00 |
| Passed | SRR207116 | 18629170.00 |
back to Summary
Mapping rate > 65.0%
| Status | Sample | Mapping Rate |
|---|
| Passed | SRR029131 | 69.23% |
| Warning | SRR029124 | 49.64% |
| Passed | SRR207111 | 87.85% |
| Warning | SRR207116 | 49.29% |
back to Summary
tRNA read share > 5.0%
| Status | Sample | tRNA Read Percentage |
|---|
| Passed | SRR029131 | 10.56% |
| Passed | SRR029124 | 17.75% |
| Passed | SRR207111 | 9.51% |
| Warning | SRR207116 | 0.93% |
back to Summary
rRNA read share < 35.0%
| Status | Sample | rRNA Read Percentage |
|---|
| Passed | SRR029131 | 0.27% |
| Passed | SRR029124 | 0.69% |
| Passed | SRR207111 | 0.02% |
| Passed | SRR207116 | 1.02% |
back to Summary
Reads mapping to unannotated regions < 35.0%
| Status | Sample | Unannotated Region Mapping Rate |
|---|
| Passed | SRR029131 | 8.76% |
| Passed | SRR029124 | 23.80% |
| Passed | SRR207111 | 8.69% |
| Passed | SRR207116 | 29.96% |
back to Summary
Read length average < 40 bases
| Status | Sample | Average Read Length |
|---|
| Passed | SRR029131 | 24.18 |
| Passed | SRR029124 | 22.42 |
| Passed | SRR207111 | 18.63 |
| Passed | SRR207116 | 27.62 |
back to Summary
>= 70.0% of reads between 15 and 50 bases
| Status | Sample | Read Percentage |
|---|
| Passed | SRR029131 | 100.00% |
| Passed | SRR029124 | 100.00% |
| Passed | SRR207111 | 99.91% |
| Passed | SRR207116 | 97.45% |
back to Summary
>= 50.0% of tRNAs with more than 20 reads
| Status | Sample | Percentage of tRNAs |
|---|
| Passed | SRR029131 | 99.60% |
| Passed | SRR029124 | 99.60% |
| Passed | SRR207111 | 99.60% |
| Passed | SRR207116 | 99.60% |
back to Summary
DESeq2 size factor differences < 3.0x
| Status | Sample | Size Factor |
|---|
| Passed | SRR029131 | 1.00 |
| Passed | SRR029124 | 1.53 |
| Passed | SRR207111 | 0.88 |
| Passed | SRR207116 | 0.88 |
back to Summary